smart-seq single-cell rna sequencing data set (Allen Institute for Brain Science)
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Smart Seq Single Cell Rna Sequencing Data Set, supplied by Allen Institute for Brain Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Do Corticosteroid Receptor mRNA Levels Predict the Expression of Their Target Genes?"
Article Title: Do Corticosteroid Receptor mRNA Levels Predict the Expression of Their Target Genes?
Journal: Journal of the Endocrine Society
doi: 10.1210/jendso/bvac188
Figure Legend Snippet: Mineralocorticoid receptor (MR) and glucocorticoid receptor (GR) messenger RNA (mRNA) levels do not predict glucocorticoid-induced leucine zipper (GILZ) mRNA expression in hippocampal glutamatergic and GABAergic neurons. Cellular MR ( Nr3c2 ), GR ( Nr3c1 ), and GILZ ( Tsc22d3 ) mRNA levels were obtained from a previously published single-cell sequencing data set of naive mouse hippocampi. All expression values are log-normalized and scaled according to the Seurat pipeline. MR and GR mRNA levels poorly predicted Tsc22d3 expression in A to F, glutamatergic neurons of the dentate gyrus (DG), CA3, and CA1 regions or in G to L, various subclasses of GABAergic neurons, that is, Vip, Sncg, and Lamp5 neurons. Data were analyzed with simple linear regression. The graphs show the 95% CI, as well as the statistical significance ( P ), slope (β), and strength ( R 2 ) of the correlations found. * P less than .05, ** P less than .01.
Techniques Used: Expressing, Sequencing
Figure Legend Snippet: Mineralocorticoid receptor (MR) and glucocorticoid receptor (GR) messenger RNA (mRNA) levels do not predict Fkbp5 expression in hippocampal glutamatergic and GABAergic neurons. Cellular MR ( Nr3c2 ), GR ( Nr3c1 ), and glucocorticoid-induced leucine zipper (GILZ) ( Tsc22d3 ) mRNA levels were obtained from a previously published single-cell sequencing data set of naive mouse hippocampi. All expression values are log-normalized and scaled according to the Seurat pipeline. MR and GR mRNA levels poorly predicted Fkbp5 expression in A to F, glutamatergic neurons of the dentate gyrus (DG), CA3, and CA1 regions, or in G to L, various subclasses of GABAergic neurons, that is, Vip, Sncg, and Lamp5 neurons. Data were analyzed with simple linear regression. The graphs show the 95% CI, as well as the statistical significance ( P ), slope (β), and strength ( R 2 ) of the correlations found. * P less than .05, ** P less than .01.
Techniques Used: Expressing, Sequencing
Figure Legend Snippet: 11βHsd1 expression weakly correlates with glucocorticoid-induced leucine zipper (GILZ) or FKBP5 messenger RNA (mRNA) expression in hippocampal glutamatergic and GABAergic neurons. Cellular 11βHSD1, GILZ, and FKBP5 mRNA levels were obtained from a previously published single-cell sequencing data set of naive mouse hippocampi. All expression values are log-normalized and scaled according to the Seurat pipeline. A to F, 11βHsd1 expression poorly predicted Tsc22d3 or Fkbp5 expression in A to F, glutamatergic neurons of the dentate gyrus (DG), CA3, and CA1 regions, or in G to L, various subclasses of GABAergic neurons, that is, Vip, Sncg, and Lamp5 neurons. Data were analyzed with simple linear regression. The graphs show the 95% CI, as well as the statistical significance ( P ), slope (β), and strength ( R 2 ) of the correlations found. * P less than .05, *** P less than .001.
Techniques Used: Expressing, Sequencing
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